Transcription of Brain Natriuretic Peptide and Atria1 Natriuretic Peptide Genes in Human Tissues.

We have compared the expression of atria1 natriuretic peptide (ANP) and brain natriuretic peptide (BNP) genes in various human tissues using a quantitative polymerase chain reaction technique. Tissues of three human subjects, obtained at autopsy, were analyzed. BNP transcripts could be detected in the central nervous system, lung, thyroid, adrenal, kidney, spleen, small intestine, ovary, uterus, and striated muscle. ANP transcripts could also be demonstrated in various human extracardiac tissues including several endocrine organs. In all periphera1 tissues, the level of both natriuretic peptide transcripts was approximately l-2 orders of magnitude lower than in cardiac ventricular tissues. This distribution is in marked contrast to the much lower level of ANP and BNP transcripts present in extracardiac rat tissues (generally less than l/1000 of ventricles). These data suggest differential expression of the two natriuretic peptide genes in cardiac and extracardiac tissues in man. Furthermore, the presence of local synthesis of ANP and BNP in various peripheral organs su gests paracrine and/or autocrine function of these natriuretic peptides

Effects of Universal Design for Learning math menus on seventh grade students

The purpose of this study was to: (a) examine the effectiveness of UDL math menus in increasing the academic engagement of seventh grade students with disabilities, (b) examine the effectiveness of UDL math menus in increasing the academic achievement of seventh grade students with disabilities, and (c) determine if seventh grade students with disabilities are satisfied with the use of UDL math menus. The research was conducted using single-subject design methodology. The study followed an ABAB alternating baseline pattern. Student academic achievement was evaluated through daily assessments, while student engagement was evaluated five times per class, approximately every fifteen minutes daily. The results of this study suggest that the use of UDL math menus may help to increase the academic engagement and academic achievement of seventh grade students with disabilities. UDL math menus were found to increase the weekly mean engagement score for students, and the weekly mean academic achievement score for all students in the first intervention phase. Results also show that all students were satisfied with the use of UDL math menus. Implications for educating students with disabilities in a resource room setting include the recommendation to utilize additional education technologies such as UDL math menus in the classroom

Separate and Different Education: A History of Women at the University of Windsor, 1920 to the Present

Although the experience of women in higher education has traditionally occupied a limited space in Canadian historiography, recent work by feminist and women\u27s historians has uncovered a rich and complex field. The field is a relatively new one, less mature than in the United States and Britain, nevertheless historians are beginning to suggest new approaches to the history of women in Canadian universities. 1 Scholars have produced several institutional studies which analyze the historical experience of women at particular universities and which establish the groundwork for modifying our understanding the history of women in higher education in Canada

Sugar additives for MALDI matrices improve signal allowing the smallest nucleotide change (A:T) in a DNA sequence to be resolved

Sample preparation for matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS) of DNA is critical for obtaining high quality mass spectra. Sample impurity, solvent content, substrate surface and environmental conditions (temperature and humidity) all affect the rate of matrix–analyte co-crystallization. As a result, laser fluence threshold for desorption/ionization varies from spot to spot. When using 3-hydroxypicolinic acid (3-HPA) as the matrix, laser fluence higher than the threshold value reduces mass resolution in time-of-flight (TOF) MS as the excess energy transferred to DNA causes metastable decay. This can be overcome by either searching for ‘hot’ spots or adjusting the laser fluence. However, both solutions may require a significant amount of operator manipulation and are not ideal for automatic measurements. We have added various sugars for crystallization with the matrix to minimize the transfer of excess laser energy to DNA molecules. Fructose and fucose were found to be the most effective matrix additives. Using these additives, mass resolution for DNA molecules does not show noticeable deterioration as laser energy increases. Improved sample preparation is important for the detection of single nucleotide polymorphisms (SNPs) using primer extension with a single nucleotide. During automatic data acquisition it is difficult to routinely detect heterozygous A/T mutations, which requires resolving a mass difference of 9 Da, unless a sugar is added during crystallization

Remy: AR Assisted Cooking

Our project introduces the concept of “detached monitoring” in a context-adaptive cooking system. The system has two parts: the Rat, a device mounted above the stove and the Hat, an augmented reality (AR) headset worn by the user. The Rat provides information about the user\u27s actions and the food being cooked. This information, combined with information from the Hat, is used to determine the user\u27s context. Instructions and status information are then embedded in the user\u27s environment via the Hat. The system was piloted with 7 participants in a kitchen setting. The results indicated that users found the tasks easier the more detached monitoring it incorporated, and, overall, found detached monitoring to be intuitive

Hypoxia Upregulates Estrogen Receptor β in Pulmonary Artery Endothelial Cells in a HIF-1α-Dependent Manner

17β-Estradiol (E2) attenuates hypoxia-induced pulmonary hypertension (HPH) through estrogen receptor (ER)-dependent effects, including inhibition of hypoxia-induced endothelial cell proliferation; however, the mechanisms responsible for this remain unknown. We hypothesized that the protective effects of E2 in HPH are mediated through hypoxia-inducible factor 1α (HIF-1α)-dependent increases in ERβ expression. Sprague-Dawley rats and ERα or ERβ knockout mice were exposed to hypobaric hypoxia for 2-3 weeks. The effects of hypoxia were also studied in primary rat or human pulmonary artery endothelial cells (PAECs). Hypoxia increased expression of ERβ, but not ERα, in lungs from HPH rats as well as in rat and human PAECs. ERβ mRNA time dependently increased in PAECs exposed to hypoxia. Normoxic HIF-1α/HIF-2α stabilization increased PAEC ERβ, whereas HIF-1α knockdown decreased ERβ abundance in hypoxic PAECs. In turn, ERβ knockdown in hypoxic PAECs increased HIF-2α expression, suggesting a hypoxia-sensitive feedback mechanism. ERβ knockdown in hypoxic PAECs also decreased expression of the HIF inhibitor prolyl hydroxylase 2 (PHD2), whereas ERβ activation increased PHD2 and decreased both HIF-1α and HIF-2α, suggesting that ERβ regulates the PHD2/HIF-1α/HIF-2α axis during hypoxia. Whereas hypoxic wild-type or ERα knockout mice treated with E2 demonstrated less pulmonary vascular remodeling and decreased HIF-1α after hypoxia compared with untreated hypoxic mice, ERβ knockout mice exhibited increased HIF-2α and an attenuated response to E2 during hypoxia. Taken together, our results demonstrate a novel and potentially therapeutically targetable mechanism whereby hypoxia, via HIF-1α, increases ERβ expression and the E2-ERβ axis targets PHD2, HIF-1α, and HIF-2α to attenuate HPH development